A型鸭肝炎病毒微基因组的构建

doi:10.11843/j.issn.0366-6964.2014.04.015

Abstract

Abstract:

In this study we constructed and identified the mini-genome of Duck Hepatitis A virus (DHAV), for analysis of the functional and structural properties of DHAV un-translated region (UTR).To construct a bicistronic of DHAV mini-genome (pRluc-fLuc)，the ORF of DHAV was replaced by firefly luciferase (fLuc) gene through enzyme digestion and the Renilla luciferase (Rluc) gene was inserted before 5′UTR.We also added the hammerhead ribozyme ahead Rluc gene and the hepatitis delta virus ribozyme behind 3′UTR.We transfected the pRluc-fLuc into DF-1 cells，the fLuc gene expression could be detected at 8 hours post transfected (h.p.t.) and with the levels peaking was at 24 h.p.t.The results showed that the DHAV mini-genome was constructed successfully and can be used for explore the role of UTR in virus transcription and translation.

CLC Number:

S852.659.6

LIANG Rui-ying，HU Wen，LI Ning，MIAO Qiu-hong，BI Zhuang-li，MENG Chun-chun，LI Chuan-feng，CHEN Zong-yan， LIU Guang-qing. Construction of the Mini-genome of Duck Hepatitis A Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 609-613.

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Construction of the Mini-genome of Duck Hepatitis A Virus

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